DNA Technology - II

Chapter 9 Biotechnology and DNA Technology

Unformatted text preview: Biology, 7e (Campbell) Chapter 20: DNA Technology and Genomics Chapter Questions 1) Plasmids are important in biotechnology because they are A) a vehicle for the insertion of foreign genes into bacteria. B) recognition sites on recombinant DNA strands. C) surfaces for protein synthesis in eukaryotic recombinants. D) surfaces for respiratory processes in bacteria. E) proviruses incorporated into the host DNA. Answer: A Topic: Concept 20.1 Skill: Knowledge 2) If you discovered a bacterial cell that contained no restriction enzymes, which of the following would you expect to happen? A) The cell would be unable to replicate its DNA. B) The cell would create incomplete plasmids. C) The cell would be easily infected and lysed by bacteriophages. D) The cell would become an obligate parasite. E) Both A and D would occur. Answer: C Topic: Concept 20.1 Skill: Comprehension Figure 20.1 3) Which enzyme was used to produce the molecule in Figure 20.1? A) ligase B) transcriptase C) a restriction enzyme D) RNA polymerase E) DNA polymerase Answer: C Topic: Concept 20.1 Skill: Comprehension 1 4) Assume that you are trying to insert a gene into a plasmid. Someone gives you a preparation of genomic DNA that has been cut with restriction enzyme X. The gene you wish to insert has sites on both ends for cutting by restriction enzyme Y. You have a plasmid with a single site for Y, but not for X. Your strategy should be to A) insert the fragments cut with X directly into the plasmid without cutting the plasmid. B) cut the plasmid with restriction enzyme X and insert the fragments cut with Y into the plasmid. C) cut the DNA again with restriction enzyme Y and insert these fragments into the plasmid cut with the same enzyme. D) cut the plasmid twice with restriction enzyme Y and ligate the two fragments onto the ends of the DNA fragments cut with restriction enzyme X. E) cut the plasmid with enzyme X and then insert the gene into the plasmid. Answer: C Topic: Concept 20.1 Skill: Application 5) What is the enzymatic function of restriction...

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