Gold Biotechnology kanamycin
Kanamycin is an aminoglycoside antibiotic often used to select for bacteria that have been successfully transformed with a plasmid conferring kanamycin resistance. Kanamycin is often used as a selective agent for the incorporation of the NPT II (APH3) gene in plant tissues. Kanamycin is very soluble in aqueous solution and is typically used in final concentrations of 50-100 µg/ml.
Aminoglycosides target the 30S ribosomal subunit resulting in an inability to read mRNA, and ultimately producing a faulty or nonexistent protein. Kanamycin is commonly used in clinical in vitro microbiological antimicrobial susceptibility tests (panels, discs, and MIC strips) against gram-positive and gram-negative microbial isolates. Medical microbiologists use AST results to recommend antibiotic treatment options for infected patients. However, even though kanamycin is a broad-spectrum antibiotic, it is mostly used against aerobic gram-negative bacteria.
TESTED AGAINST BOTH SENSITIVE AND RESISTANT CELLS AT GOLD BIOTECHNOLOGY LAB