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Investigation 9 Biotechnology answers

Investigation 9: Biotechnology - Restriction Enzyme Analysis of DNA

Cat. #AP09/112

Analysis of Eco RI Cleavage Patterns of Lambda DNA

This experiment introduces the use of restriction enzymes as a tool to digest DNA at specific nucleotide sequences. Bateriophage lambda DNA has a linear structure and 6 Eco RI recognition sites. Separation by agarose gel electrophoresis of an digest of lambda DNA will yield 6 bands (5 distinct bands, two are very close in size) corresponding to the DNA fragments.

Description

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Resources

Kit Includes: instructions, DNA samples (packaged as either Pre-aliquoted QuickStrip™ connected tubes or as individual 1.5 ml (or 0.5 ml) microcentrifuge tubes), UltraSpec-Agarose™ powder, concentrated electrophoresis buffer, FlashBlue™ DNA stain, InstaStain® Blue cards, practice gel loading solution, 1 ml pipet, and microtipped transfer pipets

All You Need: horizontal gel electrophoresis apparatus, D.C. power supply, automatic micropipets with tips, balance, microwave, hot plate or burner, pipet pump, 250 ml flasks or beakers, hot gloves, safety goggles and disposable laboratory gloves, small plastic trays or large weigh boats (for gel destaining), DNA visualization system (white light), and distilled or deionized water

Storage: Room Temperature Stable. Storage of Ready-to-Load QuickStrip™ samples in the Refrigerator is Recommended.

Interesting facts
Aldon Corporation Innovating Science IS3709 AP Biology Investigation #9 - Biotechnology: Restriction Enzyme Analysis of DNA
BISS (Aldon Corporation)
  • Students will perform the electrophoresis process on DNA samples treated with different restriction enzymes and construct a standard curve using a known DNA sample
  • While performing the process they will also determine the approximate size of the DNA fragments in the unknown samples
  • Materials provided for eight lab groups
  • Meets AP Science Practices 3 and 6, and Big Idea 3
Coordinated framework for regulation of biotechnology : hearing before the Subcommittee on Investigations and Oversight and the Subcommittee on Natural Resources, Agriculture Research, and Environment and the Subcommittee on Science, Research, and Technology of the Committee on Science and Technology, House of Representatives, Ninety-ninth Congress, second session, July 23, 1986.
Book (U.S. G.P.O.For sale by the Supt. of Docs., Congressional Sales Office, U.S. G.P.O.)
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