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Newswise — Scientists in South Dakota will examine living cells at a molecular level to find out how cells become cancerous, how viruses attack animals and humans and how plants can capture more nitrogen through a new collaborative research center called Biochemical Spatio-temporal NeTwork Resource (BioSNTR).

“Our goal is to create the infrastructure to catalyze innovation and discovery in bioscience and biotechnology, ” said Adam Hoppe, associate professor of chemistry and biochemistry at South Dakota State University and director of BioSNTR.

BioSNTR brings together plant and animal scientists, biochemists and biomedical engineers from six state universities, three tribal colleges and three private colleges along with medical researchers from Avera Research Institute and Sanford Research. Its focus on improving human health and agriculture is part of the state’s science and technology plan.

The center receives funding from the state of South Dakota and the South Dakota Experimental Program to Stimulate Competitive Research (EPSCoR) through the National Science Foundation EPSCoR Research Infrastructure Improvement Track I program.

Imaging molecular interactions of cells
Tools that let researchers track the biochemical machinery of living cells can help solve problems affecting the health of humans, animals and plants, Hoppe explained.

Through an NSF Career grant, Hoppe designed and built a unique microscope setup capable of imaging the inner molecular working of living cells.

“The main goal was to be able to see when three or more molecules are touching one another, ” he said, because that is how information is relayed. By tagging these molecules with different fluorescent colors, Hoppe’s instrument is able to visualize biochemical communication by measuring the transfer of energy when these molecules touch, bringing the tags to within about one-ten-millionth of an inch of each other.

Hoppe developed a computer program that separates the changes in color intensity that occur from this energy transfer and produces movies of when and where the molecules interact. Hoppe and other researchers then use these data to understand cellular processes ranging from what triggers the development of cancer to how viruses work.

Collaborating with researchers from the University of Nebraska Medical Center Eppley Institute for Research in Cancer and Allied Diseases, Hoppe applies this technology to study how molecular disruptions within white blood cells can lead to the development of leukemia.

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